Accounting for Within-Species Variation in Continuous Trait Evolution on a Phylogenetic Network
Abstract
Within-species trait variation may be the result of genetic variation, environmental variation, or measurement error, for example. In phylogenetic comparative studies, failing to account for within-species variation has many adverse effects, such as increased error in testing hypotheses about evolutionary correlations, biased estimates of evolutionary rates, and inaccurate inference of the mode of evolution. These adverse effects were demonstrated in studies that considered a tree-like underlying phylogeny. Comparative methods on phylogenetic networks are still in their infancy. The impact of within-species variation on network-based methods has not been studied. Here, we introduce a phylogenetic linear model in which the phylogeny can be a network to account for within-species variation in the continuous response trait assuming equal within-species variances across species. We show how inference based on the individual values can be reduced to a problem using species-level summaries, even when the within-species variance is estimated. Our method performs well under various simulation settings and is robust when within-species variances are unequal across species. When phenotypic (within-species) correlations differ from evolutionary (between-species) correlations, estimates of evolutionary coefficients are pulled towards the phenotypic coefficients for all methods we tested. Also, evolutionary rates are either underestimated or overestimated, depending on the mismatch between phenotypic and evolutionary relationships. We applied our method to morphological and geographical data from Polemonium. We find a strong negative correlation of leaflet size with elevation, despite a positive correlation within species. Our method can explore the role of gene flow in trait evolution by comparing the fit of a network to that of a tree. We find marginal evidence for leaflet size being affected by gene flow and support for previous observations on the challenges of using individual continuous traits to infer inheritance weights at reticulations. Our method is freely available in the Julia package PhyloNetworks.
Keywords: gene flow, hybridization, Polemonium, measurement error, phylogenetic regression, PGLS, REML
How to Cite:
Teo, B., Rose, J., Bastide, P., Ané, C., Teo, B., Rose, J., Bastide, P. & Ané, C., (2023) “Accounting for Within-Species Variation in Continuous Trait Evolution on a Phylogenetic Network”, Bulletin of the Society of Systematic Biologists 2(3), 1-29. doi: https://doi.org/10.18061/bssb.v2i3.8977
Rights: Benjamin Teo, Jeffrey P. Rose, Paul Bastide, Cécile Ané
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